Explore different types of Ussing Chamber Systems
Ussing chambers are powerful tools, but they are often chosen for the wrong reasons.Many researchers encounter the technique early in training and assume it applies broadly. In practice, Ussing chambers excel in specific experimental situations and perform poorly in others. This overview is meant to help researchers decide when an Ussing chamber is appropriate and…
Die Ussing-Kammer ist ein zentrales experimentelles Werkzeug zur Untersuchung von Epithelgeweben. Sie ermöglicht es, Transportprozesse, Barriereeigenschaften und elektrophysiologische Funktionen von biologischen Membranen unter kontrollierten Bedingungen zu messen. Im Gegensatz zu vielen vereinfachten In-vitro-Systemen erlaubt die Ussing-Kammer die funktionelle Analyse intakter, polarer Gewebe. Genau darin liegt ihr wissenschaftlicher Wert. Historischer Hintergrund und wissenschaftliche Einordnung Die Methode…
Ion transport across epithelial tissues plays a fundamental role in physiological processes, including nutrient absorption, waste excretion, and cellular homeostasis. To understand the underlying mechanisms governing ion movement, researchers employ specialized techniques such as the Ussing chamber, which facilitates the study of both active and passive transport. This article delves into the principles of ion…
The Ussing chamber is a classic experimental apparatus used to study the transport properties and barrier functions of epithelial tissues—surfaces that line organs such as the intestines, lungs, and kidneys. By mounting a section of live tissue between two chambers, researchers can precisely control the environment on both the “mucosal” (luminal) side and the “serosal”…
Hydrogen Sulfide Triggering Secretion This study explores how hydrogen sulfide (H₂S) triggers secretion in the intestines, focusing on the mechanisms and pathways involved. H₂S, a gasotransmitter, is known for its role in regulating various physiological processes and its potential therapeutic uses. Researchers investigated the effects of an H₂S donor compound, NaHS, on guinea-pig and human…
In the study “Neural influences on human intestinal epithelium in vitro,” researchers actively investigated how multiple neural factors importantly affect ion transport across human intestinal epithelial tissues. To achieve this goal, they actively used the EasyMount Ussing Chamber system from Physiologic Instruments, which effectively enabled them to mount isolated intestinal tissue samples between two chambers,…
Researchers in the study titled “Effects of Food Components That Activate TRPA1 Receptors on Mucosal Ion Transport in the Mouse Intestine” used the EasyMount Ussing Chamber system from Physiologic Instruments. They sought to examine multiple dietary components and their effects on ion transport across the intestinal mucosa. The team used this apparatus to mount isolated…
In the study “Modulation of the Respiratory Epithelium Physiology by Flavonoids—Insights from 16HBEσ Cell Model,” researchers actively examined how specific flavonoids—kaempferol, luteolin, as well as naringenin—effect the physiology of human bronchial epithelial cells. Researchers sought to understand how these compounds importantly influence multiple aspects of cell behavior. They concentrated on cell metabolic activity, proliferation, epithelial…
The Ussing chamber, also known as the Ussing-Müller chamber, is a widely used tool in physiology and cell biology research. The chamber is used to measure the electrical properties of biological tissues or cells in order to study the transport of ions and molecules across biological membranes. In cancer research, Ussing chambers have been used…